Date of Award
Spring 6-14-2013
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Physics
First Advisor
Eric Landahl, Ph.D.
Second Advisor
Jesus Pando, Ph.D.
Third Advisor
Anuj Sarma, Ph.D.
Abstract
Small Angle X-Ray Scattering (SAXS) is a rapidly developing technique for determining the structures of proteins in solution. This thesis presents new solution structures of two proteins:cold denatured equine cytochrome-c,and mitochondrial rho (miro).Cytochrome-cis a modelsystem for protein folding and stability studies;this work demonstrates the interplay of thermodynamic forces in determining the global shape of unfolded proteins.Whereas denatured cytochrome-cis not able to form crystals in principle,miro is a newly purified protein of which no crystal structure is availablein any conditions.We have used our SAXS data to verify conjectures for miro's structure based solely on protein sequence analysis.
Recommended Citation
Banks, Anthony, "Structural Studies of Proteins in Solution: Cytochrome-C and Mitochondrial RHO" (2013). College of Science and Health Theses and Dissertations. 47.
https://via.library.depaul.edu/csh_etd/47