College of Science and Health Theses and Dissertations

Date of Award

Spring 6-14-2013

Degree Type


Degree Name

Master of Science (MS)



First Advisor

Eric Landahl, Ph.D.

Second Advisor

Jesus Pando, Ph.D.

Third Advisor

Anuj Sarma, Ph.D.


Small Angle X-Ray Scattering (SAXS) is a rapidly developing technique for determining the structures of proteins in solution. This thesis presents new solution structures of two proteins:cold denatured equine cytochrome-c,and mitochondrial rho (miro).Cytochrome-cis a modelsystem for protein folding and stability studies;this work demonstrates the interplay of thermodynamic forces in determining the global shape of unfolded proteins.Whereas denatured cytochrome-cis not able to form crystals in principle,miro is a newly purified protein of which no crystal structure is availablein any conditions.We have used our SAXS data to verify conjectures for miro's structure based solely on protein sequence analysis.

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